Why is it that experiments that are supposed to be easy and straight forward always find a way to not work out?
For example, my co-IPs. I did them about a month ago and all was good. I got the result I wanted and did a happy dance. It was a good day indeed. So now I need to go through the formality of confirming the results. I do the co-IPs again on another set of cell lysates. I made sure I did it pretty much the same as with the first set. Well apparently not. It seems that I have a lot of background in lanes that are supposed to have nothing. So frustrating! This was supposed to be a simple confirmation experiment. Now for the trouble shooting. I normally wouldn't be so angry about having to re-do an experiment since this is science afterall and it's expected but I pretty much only have this experiment and one more (hopefully simple) experiment left to do and I just want to get them done. It just seems like the science gods are against me.
Oh and on that note I ordered some stuff for an experiment like a week and a half ago and I still don't have it. I went to our departmental order desk to see what's up with the order. It seems that the reason I don't have it yet is because they only ordered it on Friday - NINE DAYS AFTER I SENT THE ORDER TO THEM!!! Hello?!? I didn't order it because I need it next month I ordered it because I need it now. I swear the world doesn't want me to get my degree.